Scientist
Glen / Glenda (as Daniel Davis)
Inspector Warren
Dr. Alton / Narrator
Barbara
Alan / Anne
Johnny (as Charles Crafts)
Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks)
Man with Hat and Receding Hairline (uncredited)
Woman in Nightmare (uncredited)
The Devil / Glen's Father (uncredited)
(uncredited)
Miss Stevens (uncredited)
Minor Role (uncredited)
Man in Nightmare (uncredited)
Judge (uncredited)
Patrick / Patricia (uncredited)
Man at Transvestite's Suicide (uncredited)
Sheila, Glen's Sister (uncredited)