Eddie Valiant
Judge Doom
Dolores
Roger Rabbit / Benny The Cab / Greasy / Psycho (voice)
Jessica Rabbit (voice) (uncredited)
Marvin Acme
R.K. Maroon
Lt. Santino
Baby Herman (voice)
Jessica's Performance Model
Raoul
Augie
Angelo
Arthritic Cowboy
Soldier
Stretch
Editor / Gorilla (voice)
Kid #1
Kid #2
Kid #3
Blonde Starlet
Forensic #1
Forensic #2
Mailman
Newscaster
Conductor
Teddy Valiant
Mrs. Herman (voice)
Betty Boop (voice)
Daffy Duck / Tweety / Bugs Bunny / Sylvester / Porky Pig (voice)
Donald Duck (voice)
Hippo (voice)
Yosemite Sam / Foghorn Leghorn (voice)
Smart Ass (voice)
Stupid (voice)
Wheezy / Lena Hyena (voice)
Mickey Mouse (voice)
Minnie Mouse / Birds (voice)
Mr. Toad (voice)
Droopy (voice)
Bullet #1 (voice)
Bullet #2 (voice)
Bullet #3 (voice)
Singing Sword (voice) (archive sound)
Goofy / Big Bad Wolf (voice)
Pinocchio (voice)
Woody Woodpecker
Additional Voices (voice) (uncredited)
Mime (uncredited)
Dipped Shoe (voice) (uncredited)
Jessica Rabbit (singing voice) (uncredited)
Mooing Cow / Flying Witch (voice) (uncredited)
Dumbo (voice) (uncredited)
Ink and Paint Club Patron (uncredited)