Basil of Baker Street / Bartholomew (voice)
Professor Ratigan (voice)
Dr. David Q. Dawson / Thug Guard (voice)
Olivia Flaversham (voice)
Fidget (voice)
Mrs. Judson (voice)
The Mouse Queen (voice)
Mr. Flaversham (voice)
Sherlock Holmes (voice)
Bar Maid (voice)
Citizen / Thug Guard (voice)
Thug Guard (voice)
Thug Guard (voice)
Toby the Dog / Felicia the Cat (voice) (uncredited)
Dr. Watson (archive sound)
Lady Mouse (voice)
Miss Kitty Mouse (voice) (uncredited)